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KMID : 0903519910340040386
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1991 Volume.34 No. 4 p.386 ~ p.392
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Production of propylene oxide from propene by a methanotroph , Methylosinus trichosporium OB3b
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Abstract
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Whole cells of Methylosinus trichosporium OB3b, the obligate methylotroph, were used to produce propylene oxide from propane. This strain has methane monooxygenase, which catalyzes the conversion methane to methanol and can catalyze also the conversion propane to propylene oxide. Optimal condition for the production of propylene oxide was investigated in resting-whole cell system. The optimal pH and temperature was 7.0 and 35℃ respectively. The end product, propylene oxide, didn´t inhibit the production of propylene oxide and was not further metabolized in reaction mixture. The addition of methane metabolites (methanol, formaldehde and formic acid) to the reaction mixture stimulated formation of propylene oxide by 3∼4 times, and methanol was the most effective especially. Under the optimal conditions, the 14.2 mM of propylene oxide was produced after incubation of 60 min. and the conversion ratio of propane to propylene oxide was approximately 8%.
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